MACHEREY-NAGEL

n Fast isolation of microbial DNA and RNA from filtered samples n Minimized downstream inhibition for reliable results n Automation friendly Reliable detection of microorganisms in water and air samples Bioanalysis MACHEREY?NAGEL NucleoMag ® DNA /RNA Water MACHEREY?NAGEL GmbH & Co. KG \267 Neumann-Neander ww DE / International: T F E-Mail: info@mn-net.com CH: T F E-Mail: sales-ch@mn-net.com FR: T F E-Mail: sales-fr@mn-net.com \037\036\035\034\033\032\032\031 \030\027\026 MACHEREY?NAGEL GmbH & Co. KG \267 Neumann-Neander w w DE / International: T F E-Mail: info@mn-net.com CH: T F E-Mail: sales-ch@mn-net.com FR: T F E-Mail: sales-fr@mn-net.com \037\036\035\034\033\032\032\031 \030\027\026 DNA and RNA purification from water and air Microbial nucleic acids from water and air The new NucleoMag ® DNA/RNA Water enables manual or au? tomated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. NucleoMag ® DNA/RNA is compatible with a variety of filters and filtration systems, including conventional round filters (e. g., 25 mm to 47 mm) from various materials as well as with cartridge filters (such as Sterivex™). This makes NucleoMag ® DNA/RNA Water ideal for microbial metagenomics, environ? mental microbiology as well as for estimation of water quality through determination of the microbial load of the sample. Product at a glance NucleoMag ® DNA/RNA Water Technology Magnetic beads Format Highly reactive superparamagnetic beads Processing Manual or automated Sample material Water or air samples Maximum amount of starting material 10–1000 mL per preparation Fragment size 300 bp–approx. 50 kbp Elution volume 50–200 ?L Preparation time 40 min for KingFisher ® Flex (excl. filtration and sample lysis) Theoretical binding capacity* 0.4 ?g/?L beads Procedure Filter thewater sample Transfer the lterHomo\fenize the lter Transfer lysate P\bre n\bcleic acid Bind-wash-el\bte Application data CT 0 30 25 20 15 10 5 Creek River 1 River 2 Water reservoir LakeSeaColl. water Pond Pool Air Efficient detection for different water and air samples Various water samples and an air sample were filtered and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag ® DNA/RNA Water kit. CT 0 25 20 15 10 5 Cellulose mixed esters Cellulose acetate Cellulosenitrate RegeneratedCellulose Poly?carbonate ThermoFilter Sterivex™Merck Compatibility with different filtration systems A qPCR was performed with nucleic acids isolated from round filters and a filtration cartridge system, demonstrating reliable results across different fil ? tration systems. Ordering information Specification Pack ofREF NucleoMag ® DNA/RNA Water 1 × 96 / 4 × 96 preps 744220.1 / .4 NucleoSpin® Bead Tubes Type A 50 preps 740786.50 NucleoSpin® Bead Tubes Type A 5 mL 50 preps 740799.50 MN Bead Tube Holder 1740469 MN Bead Tube Holder 5 mL 1740459 Trademarks: NucleoMag and NucleoSpin are registered trademarks of MACHEREY ?NAGEL GmbH & Co KG KingFisher is a registered trademark of Thermo Fisher Scientific · Sterivex is a trademark of Merck KGaA. Mag © John Smith – fotolia.com KATEN300209 Flyer NucleoMag ® DNA?RNA Water Kit en1 / 2 / 0 / 04.2019 DD · Printed in Germany US: Tel.: +1 484 821 0984 Fax: +1 484 821 1272 E-Mail: sales-us@mn-net.com MACHEREY?NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany www.mn-net.com www.mn-net.com DE / International: Tel.: +49 24 21 969-0 Fax: +49 24 21 969-199 E-mail: info@mn-net.com CH: Tel.: +41 62 388 55 00 Fax: +41 62 388 55 05 E-mail: sales-ch@mn-net.com FR: Tel.: +33 388 68 22 68 Fax: +33 388 51 76 88 E-mail: sales-fr@mn-net.com

Smart photometry NANOCOLOR UV /VIS II Guide de démarrage rapide MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany France: MACHEREY-NAGEL EURL Tel.: +33 388 68 22 68 Fax: +33 388 51 76 88 E-mail: sales-fr@mn-net.com Switzerland: MACHEREY-NAGEL AG Tel.: +41 62 388 55 00 Fax: +41 62 388 55 05 E-mail: sales-ch@mn-net.com Germany and international: Tel.: +49 24 21 969-0 Fax: +49 24 21 969-199 E-mail: info@mn-net.com MACHEREY-NAGEL EN ISO 9001: 2008 CERTIFIED

Analyses de l’eau MACHEREY-NAGEL Système d’analyse NANOCOLOR® MACHEREY-NAGEL SARL 1, rue Gutenberg 67722 Hoerdt ? France www.mn-net.com Tel. : +33 388 68 22 68 Fax : +33 388 51 76 88 E-mail : sales-fr@mn-net.comDE / International : Tel. : +49 24 21 969-0 Fax : +49 24 21 969-199 E-mail : info@mn-net.comCH : Tel. : +41 62 388 55 00 Fax : +41 62 388 55 05